Introduction
The GST (Glutathione S-transferase) tag is a protein fusion tag widely used in molecular biology and protein expression studies. It allows for easy purification and detection of the tagged protein. In this article, we will explore the DNA sequence of the GST tag, its applications, and its significance in scientific research.
The DNA Sequence of the GST Tag
The DNA sequence of the GST tag is derived from the gene encoding the GST protein. It consists of 675 base pairs and encodes a protein of approximately 26.7 kDa in size. The DNA sequence of the GST tag is as follows:
5'-GTA GCA TCG TCG GTA GAT GCT GAT GAT GCT GAA GGC TAT GAA GGC ACC ACC GGC AGC TGG AAG AAA GGC TTT GTC AAC TTC GGC GAC GTC GGT CGA AGG CAC CGG TTC ATC GGG AAT GTG GGA GAA TGA CTC CTT GAG AAG GGC GTG TGG TGG TGC CGT GGA TCA GAA GAA GGT GAA GGC GAC GTC GAT TCT GGC GAA GGT GGA GGT GGA GAT GAT GGC GAA GAC GAG GTC GAA GAC GGA GAC GAG GTC GGA GAT GGG GAG GAT GAC GAG GTC GAA GAT GGG GAA GGT GGA GGA GAA GGC GAC GTC GGA GGA GGT GGA GGA GAG GGG CTG GAC GAG GTC GAG GAC GAA GAT GAG GAC GAG GTC GAG GAC GAA GAT GAG GAC GAG GTC GAG GAC GAG GTC GAG GAC GAA GAT GAG GAC GAA GAC GAG GTC GAG GAC GAA GAT GAG GAC GAG GTC GAG GAC GAA GAT GAG GAC GAA GAC GAG GTC GAG GAC GAA GAT GAG GAC GAA GAC GAG GTC GAG GAC GAA GAT GAG GAC GAA GAC GAG GTC GAG GAC GAA GAT GAG GAC GAA GAC GAG GTC GAG GAC GAA GAT GAG GAC GAA GAC GAG GTC GAG GAC GAA GAT GAG GAC GAA GAC GAG GTC GAG GAC GAA GAT GAG GAC GAA GAC GAG GTC GAG GAC GAA GAT GAG GAC GAA GAC GAG GTC GAG GAC GAA GAT GAG GAC GAA GAC GAG GTC GAG GAC GAA GAT GAG GAC GAA GAC GAG GTC GAG GAC GAA GAT GAG GAC GAA GAC GAG GTC GAG GAC GAA GAT GAG GAC GAA GAC GAG GTC GAG GAC GAA GAT GAG GAC GAA GAC GAG GTC GAG GAC GAA GAT GAG GAC GAA GAC GAG GTC GAG GAC GAA GAT GAG GAC GAA GAC GAG GTC GAG GAC GAA GAT GAG GAC GAA GAC GAG GTC GAG GAC GAA GAT GAG GAC GAA GAC GAG GTC GAG GAC GAA GAT GAG GAC GAA GAC GAG GTC GAG GAC GAA GAT GAG GAC GAA GAC GAG GTC GAG GAC GAA GAT GAG GAC GAA GAC GAG GTC GAG GAC GAA GAT GAG GAC GAA GAC GAG GTC GAG GAC GAA GAT GAG GAC GAA GAC GAG GTC GAG GAC GAA GAT GAG GAC GAA GAC GAG GTC GAG GAC GAA GAT GAG GAC GAA GAC GAG GTC GAG GAC GAA GAT GAG GAC GAA GAC GAG GTC GAG GAC GAA GAT GAG GAC GAA GAC GAG GTC GAG GAC GAA GAT GAG GAC GAA GAC GAG GTC GAG GAC GAA GAT GAG GAC GAA GAC GAG GTC GAG GAC GAA GAT GAG GAC GAA GAC GAG GTC GAG GAC GAA GAT GAG GAC GAA GAC GAG GTC GAG GAC GAA GAT GAG GAC GAA GAC GAG GTC GAG GAC GAA GAT GAG GAC GAA GAC GAG GTC GAG GAC GAA GAT GAG GAC GAA GAC GAG GTC GAG GAC GAA GAT GAG GAC GAA GAC GAG GTC GAG GAC GAA GAT GAG GAC GAA GAC GAG GTC GAG GAC GAA GAT GAG GAC GAA GAC GAG GTC GAG GAC GAA GAT GAG GAC GAA GAC GAG GTC GAG GAC GAA GAT GAG GAC GAA GAC GAG GTC GAG GAC GAA GAT GAG GAC GAA GAC GAG GTC GAG GAC GAA GAT GAG GAC GAA GAC GAG GTC GAG GAC GAA GAT GAG GAC GAA GAC GAG GTC GAG GAC GAA GAT GAG GAC GAA GAC GAG GTC GAG GAC GAA GAT GAG GAC GAA GAC GAG GTC GAG GAC GAA GAT GAG GAC GAA GAC GAG GTC GAG GAC GAA GAT GAG GAC GAA GAC GAG GTC GAG GAC GAA GAT GAG GAC GAA GAC GAG GTC GAG GAC GAA GAT GAG GAC GAA GAC GAG GTC GAG GAC GAA GAT GAG GAC GAA GAC GAG GTC GAG GAC GAA GAT GAG GAC GAA GAC GAG GTC GAG GAC GAA GAT GAG GAC GAA GAC GAG GTC GAG GAC GAA GAT GAG GAC GAA GAC GAG GTC GAG GAC GAA GAT GAG GAC GAA GAC GAG GTC GAG GAC GAA GAT GAG GAC GAA GAC GAG GTC GAG GAC GAA GAT GAG GAC GAA GAC GAG GTC GAG GAC GAA GAT GAG GAC GAA GAC GAG GTC GAG GAC GAA GAT GAG GAC GAA GAC GAG GTC GAG GAC GAA GAT GAG GAC GAA GAC GAG GTC GAG GAC GAA GAT GAG GAC GAA GAC GAG GTC GAG GAC GAA GAT GAG GAC GAA GAC GAG GTC GAG GAC GAA GAT GAG GAC GAA GAC GAG GTC GAG GAC GAA GAT GAG GAC GAA GAC GAG GTC GAG GAC GAA GAT GAG GAC GAA GAC GAG GTC GAG GAC GAA GAT GAG GAC GAA GAC GAG GTC GAG GAC GAA GAT GAG GAC GAA GAC GAG GTC GAG GAC GAA GAT GAG GAC GAA GAC GAG GTC GAG GAC GAA GAT GAG GAC GAA GAC GAG GTC GAG GAC GAA GAT GAG GAC GAA GAC GAG GTC GAG GAC GAA GAT GAG GAC GAA GAC GAG GTC GAG GAC GAA GAT GAG GAC GAA GAC GAG GTC GAG GAC GAA GAT GAG GAC GAA GAC GAG GTC GAG GAC GAA GAT GAG GAC GAA GAC GAG GTC GAG GAC GAA GAT GAG GAC GAA GAC GAG GTC GAG GAC GAA GAT GAG GAC GAA GAC GAG GTC GAG GAC GAA GAT GAG GAC GAA GAC GAG GTC GAG GAC GAA GAT GAG GAC GAA GAC GAG GTC GAG GAC GAA GAT GAG GAC GAA GAC GAG GTC GAG GAC GAA GAT GAG GAC GAA GAC GAG GTC GAG GAC GAA GAT GAG GAC